PerkinElmer

Cell culture plates

PerkinElmer’s CellCarrier™ Ultra and CulturPlates™ are specifically designed to promote cell attachment and growth. These microplates are appropriate for now only cell culture, but also imaging (CellCarrier Ultra) as well as fluorescence and luminescence assays (CulturPlate)

Additionally, PerkinElmer offers a variety of coatings that can be applied to a multitude of our plates to promote cell growth.

  • Tissue-culture (TC) treatment allows for cell attachment and binding to the bottom surface of the microplate and is used for assays that use adherent cell lines.
  • Poly-D-Lysine (PDL) treatment enhances cell attachment and binding and is used when working with cells that are difficult to attach and when wash steps are needed.
  • Collagen (COL) treatment enhances cell attachment and proliferation and is used with working with keratinocytes and hepatocytes, specifically.
  • Ultra-low attachment (ULA) treatment allows for further reduction of non-specific binding and is used in cell colony high content screening assays, 3D cultures and other imaging applications.

Genomax Technologies suppliers PerkinElmer life science research reagents and consumables in Singapore. If you have any questions, please contact info@genomax.com.sg.

Microplate selection by application

Custom Microplate services

PerkinElmer offers a range of custom microplate services, including:

  • Bulk ordering and special packaging
  • Fast and flexible plate barcoding
  • Biological plate coating (including poly-D-lysine, collagen, gelatin, streptavidin coating, antibody coating, and other coatings on request)
  • Custom tissue culture-treatment
  • Custom high protein binding treatment
  • Custom sterilization of microplates
  • Other microplate treatments, if you are interested in custom microplate services, please contact info@genomax.com.sg.

ATPlite luminescence assays

ATPlite™ is an Adenosine Triphosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence assay is the alternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds. Cell viability assays are used to quantify the number of viable cells and are usually used in cell-based assays to access to cell proliferation and cytotoxicity.

Features and Benefits of ATPlite:

  • Long-lived luminescent signal: half-life (t1/2) greater than 5 hours, depending on cell type and medium
  • Rapid: results generated in 15 – 25 minutes
  • Simple and reproducible: no separation steps; only two reagent additions
  • Homogeneous assay: no cell harvesting or centrifugation required
  • Sensitive: down to 5 cells in 100µL medium (derived from CHO and HL-60 cells in 100µL medium)
  • Wide linear dynamic range: =105 (as derived from CHO and HL-60 cells).

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ATPlite

ATPlite 1step

Signal stability ​
(Half-life)​

>  4 hours​

30 min ​

Initial signal intensity
(relative intensity)​

Less bright​

Brightest ​

Remarks​

Longest signal half-life àfor batch processing of samples​

Shortest half-life  à for continuous processing of samples​

True 1 step system = More suited to HTS​

Read more: Brochure on Luminescence Assays from PerkinElmer

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