Membrane-based arrays for multiplexed protein detection and profiling
Semi-Quantitative, Sandwich-Based Cytokine Arrays
Cytokine arrays are immunoassays similar to ELISA but capable of simultaneously measuring the levels of many proteins. Many biological processes such as apoptosis, inflammation, angiogenesis, immune response, and migration often accompany changes of cytokine expression levels. Because of the extensive cross-talk between cytokines, a complete analysis of biological responses and functions must be obtained through multiplex assays.
Cytokine arrays allow a much broader view of protein activity than can be obtained with multiple single-target ELISAs, immunoprecipitations, or western blots, which may be time consuming and can require specialized equipment. Thus, cytokine arrays are a rapid, sensitive, and cost-effective alternative method to detect multiple proteins in a single liquid sample like serum, plasma, cell culture supernatant, lysates, or other biological fluids.
Types of Cytokine Arrays
Membrane Cytokine Arrays
RayBiotech’s Membrane-Based Cytokine Arrays (C-Series) enable multiplex protein detection across a wide variety of sample types and species. Processed similarly to a western blot with a chemiluminescent readout, you can explore biological processes like apoptosis, inflammation, angiogenesis, and the immune response with our sandwich-based arrays.
Regulation of cellular processes by cytokines is a complex, dynamic process, often involving multiple proteins. Cytokine array screening improves the chances for discovering key factors, disease mechanisms or biomarkers related to cytokine signaling. The RayBio® Human Cytokine Array C5 detects 80 cytokines and was used to identify GM-CSF as a cytokine secreted by mesenchymal-like breast cancer cells that activate macrophages and promote metastasis.
Features of C-Series Arrays
- Easy to use, no training required
- High specificity (sandwich antibody pair used)
- High sensitivity (pg/ml levels)
- Compatible with any chemiluminescent imaging system
- Results obtained in 1 day
- Highly published
Glass Slide Cytokine Arrays
RayBiotech’s Glass-slide based Cytokine Arrays (G-Series) also utilize the sandwich immunoassay principle but require much less sample than the membrane-based arrays. The capture antibodies are spotted on 75mm x 25mm glass slide solid supports and a corresponding biotinylated detection antibody is utilized for resolving fluorescent signals. Each slide is spotted with either 8 or 16 identical antibody arrays (also called “subarrays”). The G-series cytokine arrays are semi-quantitative, glass-based arrays that measure relative protein expression levels across samples or experimental conditions.
RayBio® Glass Slide-based Antibody Arrays (G-Series) were introduced as a “second generation” array platform after the chemiluminescent membrane arrays (C-Series). The G-Series uses a 75mm x 25mm glass chip platform which delivers easier handling along with the use of much lower sample volumes. The glass slides also utilize a fluorescent signal readout, allowing a wider dynamic detection range than can be achieved by chemiluminescence.
The G-Series cytokine arrays allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors, and other proteins from any biological fluid.
Why RayBiotech Cytokine Arrays?
- Manufacturer of cytokine array kits from the USA
- Highly responsive technical support
- Largest validated antibody pair catalogue
- Use RayBiotech ELISAs for confirmation of array data
- Customizable – create a custom array from our list of targets
- ISO certified and validated for exceptional reproducibility
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