G-Series: Glass Slide Antibody Arrays

Semi-Quantitative, Sandwich-based, Glass Slide Antibody Arrays

Antibody arrays allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid.

RayBio® Glass Slide-based Antibody Arrays (G-Series) were introduced as a “second generation” array platform after the chemiluminescent membrane arrays (C-Series). The antibody arrays simultaneously measures the expressions of multiple proteins. The G-Series uses a 75mm x 25mm glass chip platform which delivers easier handling along with the use of much lower sample volumes.

The glass slides also utilizes a fluorescent signal readout, allowing a wider dynamic detection range than can be achieved by chemiluminescence. G-Series Antibody Arrays are particularly well-suited for projects with very limited sample volumes (as little as 10 μl per array) and large numbers of samples. Dozens of arrays can be processed per day by a single technician. Furthermore, the slide chamber assembly is compatible with many automated array processing systems, allowing high-throughput studies to be performed with very little hands-on time.

Please contact Genomax for the walk-through of the technology. 

G-Series Antibody Arrays utilize the sandwich-ELISA design principle. In this assay, capture antibodies are printed in multiple identical arrays on a standard-sized histology slide. After a blocking step, samples are incubated with the arrays. Nonspecific proteins are then washed off, and the arrays are incubated with a cocktail of biotinylated detection antibodies, followed by a streptavidin-conjugated fluor. Signals are visualized using a fluorescence laser scanner.