Western blotting supplies for sensitive and consistent detection of proteins

Genomax Technologies suppliers blotting reagents from BioLegend and PerkinElmer in Singapore. For more information, contact This email address is being protected from spambots. You need JavaScript enabled to view it..



BioLegend

Western blotting is a key tool in life science research that is used to separate and identify specific proteins from complex mixtures. BioLegend has developed a comprehensive selection of highly specific antibodies, ready-to-use buffers, and protein ladders. BioLegend provides an extensive selection of purified and conjugated antibodies against human, mouse, and rat soluble proteins. The antibodies can be used in a variety of applications including ELISA, flow cytometry, western blotting, and functional assays.



Browse by product types:

1. Antibodies

  • Primary Antibodies - To detect your protein of interest, we provide highly specific primary antibodies validated and quality-tested by western blotting. Our antibodies cover a wide range of targets across cell biology, neuroscience, immunology, and other key signaling pathways. These come in either a purified, unconjugated format or directly conjugated to HRP.
  • Secondary Reagents - Secondary reagents target the primary antibody and are conjugated to provide visualization of the protein of interest. For western blotting, these reagents are conjugated to HRP which is used as a reporter enzyme.



2. Buffers for protein extraction, gel loading and running, and detection

Western-Ready™ Buffers - Try the complete set of Western-Ready™ buffers for every step of your workflow, including protein extraction, gel loading and running, and detection. 

description

USE

Western-Ready™ MES SDS-PAGE Running Buffer (10X)

Running buffer for resolving small-to-medium-sized proteins on Bis-Tris SDS page gels.

Western-Ready™ Protein Sample Loading Buffer (5X)

Used for the preparation of samples for non-reducing and reducing SDS-PAGE100 reactions.

Western-Ready™ Rapid Protein Extraction Buffer Stringent lysis buffer to extract proteins from all subcellular compartments.
Western-Ready™ TBS Tween-20 Buffer (20X) Buffer for blocking, washing, and antibody dilution. Formulated to reduce non-specific binding.
Western-Ready™ Transfer Buffer (10X) Buffer formulated for western blot protein transfer onto PVDF or nitrocellulose membranes.



3. Chemiluminescent substrates

PerkinElmer

Choose Western Lightning ONE™ chemiluminescent substrates for western blotting to validate your CRISPR experiments, in vivo imaging studies, and more.

Learn more: The Power of Western Lightning ONE from PerkinElmer



Features of Western Lightning™ ONE

  • One bottle, premixed, ready-to-use solution for ease of use
  • High signal-to-noise ratio
  • Stable at room temperature for 1 year
  • Three levels of sensitivity from mid-picograms to low femtograms, for all your detection needs
  • Consistent results by avoiding pipetting errors and potential contamination, for increased confidence
  • Compatible with all protein imaging technology chemiluminescent imagers and X-ray film
  • Suitable for different types of membranes and blocking reagents



 

4. Transfer Membranes 

The recommended membranes are the Protran™ nitrocellulose membranes for high retention of protein samples in chemiluminescent detection assays. Available in two membrane pore sizes: 0.2 µm or 0.45 µm, Protran™ nitrocellulose transfer membranes offer:

  • Protran 100% pure nitrocellulose membranes, with no cellulose acetate added, ensure the highest binding capacity possible.
  • High retention of small or large proteins: 0.2 µm pore size membranes ensures high retention of small samples/low molecular weight proteins below 20 kD by reducing “blowthrough”, whereas 0.45 µm pore size membranes are ideal for larger molecular weight samples.
  • No methanol pre-wetting step: prior to transfer, the membrane is wet in water, and then in the transfer buffer.
  • No other pre-treatment steps are necessary.

 

Contact us atTel: 65-6777 1886Fax: 65-6777 3090
Send us a messageinfo@genomax.com.sg
Visit us 237 Pandan Loop, #06-03 Westech Building, Singapore 128424