Invivoscribe LymphoTrack Assays

LymphoTrack Assays (RUO)

Invivoscribe’s Research Use Only (RUO) clonality assays detect clonal populations in a few easy steps. These steps include PCR amplification of the Immunoglobulin or T-cell receptor genes of interest, followed by detection with non-denaturing polyacrylamide gels, capillary electrophoresis, or next-generation sequencing using an Illumina MiSeq^®, Thermo Fisher Scientific^® Ion S5^® or PGM^® instrument. Here in this article we are discussing about LymphoTrack^® Assay kits that are designed for the identification of gene rearrangements in hematologic samples utilizing next-generation sequencing (NGS) technologies. Invivoscribe’s LymphoTrack^® Assay kits offer significant improvements over conventional clonality testing methods, by providing the distribution of gene rearrangements, DNA sequences, the mutational status (IGH only), and the ability to track gene rearrangements all with the same workflow.

Key Benefits:

One-step PCR for amplicon and library generation
Identify and assess mutation status of B- and T-cell gene rearrangements
Sequence amplicons from any LymphoTrack^® kit together
Included bioinformatics software for easy analysis and interpretation
Same reagents for clonality, somatic hypermutation (SHM), minimal residual disease (MRD) testing, and tracking/monitoring of immunotherapy constructs

Primers included in the master mixes are designed with Illumina^®adapters and indices (8 or 24 indices configurations for a total possible 24 or *48 unique indices [*FR1 only] per framework kits) or Thermo Fisher adapters and indices (12 indices per framework kits). By offering multiple it configurations (8-or-24 indices for MiSeq^®, 12 for Ion S5/PGM^®), Invivoscribe provides laboratories the ability to choose the optimal kit for your sample throughput and read-depth requirements. Testing costs can be reduced by sequencing in a single run, with the possibility to combine: a) samples with up to 48 different indices and b) amplicons from other LymphoTrack^® Assays.

For Research Use Only (RUO) reagents and controls, not for diagnostic purposes.